TF Info Page for KLF5 (C2H2 ZF)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro Binds as a monomer and as a dimer (PMID: 25575120).

Description

Description: Kruppel like factor 5 [Source:HGNC Symbol;Acc:HGNC:6349]
Entrez Summary This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Ensembl ID: ENSG00000102554
External Link: CisBP
Interpro IPR007087; IPR015880;
Protein Domain: ENSP00000366915
Protein Domain: ENSP00000440407
Domain:
Protein: ENSP00000366915DBD: C2H2 ZF Containing ProteinsOther:
Protein: ENSP00000440407DBD: C2H2 ZF Containing ProteinsOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:16595680
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:16595680
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
ChIP-seqJASPARDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHOMERDirect
HT-SELEXYin2017Inferred - KLF3 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF3 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF3 (84% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF3 (84% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
ChIP-seqSchmitges2016Inferred - KLF1 (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (83% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF1 (83% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF1 (83% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (82% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (82% AA Identity, Homo sapiens)
PBMBarrera2016Inferred - KLF1 (82% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF12 (82% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (82% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF12 (82% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Klf12 (82% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - KLF4 (82% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF4 (82% AA Identity, Homo sapiens)
PBMBadis09Inferred - Klf7 (82% AA Identity, Mus musculus)
HT-SELEXNitta2015Inferred - luna (82% AA Identity, Drosophila melanogaster)
ChIP-seqJASPARInferred - Klf1 (82% AA Identity, Mus musculus)
ChIP-seqSchmitges2016Inferred - KLF12 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF12 (82% AA Identity, Homo sapiens)
ChIP-seqNajafabadi2015Inferred - KLF4 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (82% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF4 (82% AA Identity, Homo sapiens)
ChIP-seqhmCHIPInferred - Klf4 (82% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - Klf4 (82% AA Identity, Mus musculus)
ChIP-seqSchmitges2016Inferred - KLF7 (82% AA Identity, Homo sapiens)
MiscHOMERInferred - Klf1 (82% AA Identity, Mus musculus)
MiscHocoMocoInferred - KLF4 (82% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF2 (80% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF2 (80% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF2 (80% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - KLF6 (79% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - KLF6 (79% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - KLF6 (79% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF6 (79% AA Identity, Homo sapiens)
PBMDREAM_contestInferred - Klf8 (78% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - KLF8 (78% AA Identity, Homo sapiens)
MiscHocoMocoInferred - KLF8 (78% AA Identity, Homo sapiens)
PBMCEPDInferred - klf-3 (71% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution