TF Info Page for POU4F3 (Homeodomain; POU)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: POU class 4 homeobox 3 [Source:HGNC Symbol;Acc:HGNC:9220]
Entrez Summary This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]
Ensembl ID: ENSG00000091010
External Link: CisBP
Interpro IPR000327; IPR001356; IPR009057; IPR010982; IPR013847; IPR017970;
Protein Domain: ENSP00000230732
Domain:
Protein: ENSP00000230732DBD: Homeodomain,POUOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:15465029
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:15465029
GO:0001228
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IDA - PMID:23805044
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:9506947
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
PBMBarrera2016Direct
PBMBarrera2016Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
PBMBerger08Inferred - Pou4f3 (100% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - POU4F1 (96% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - POU4F1 (96% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - POU4F1 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU4F1 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU4F1 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU4F2 (96% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - POU4F2 (96% AA Identity, Homo sapiens)
MiscHocoMocoInferred - POU4F2 (96% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - NEMVEDRAFT_v1g160868 (88% AA Identity, Nematostella vectensis)
PBMZoo_01Inferred - unc-86 (83% AA Identity, Caenorhabditis elegans)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution