TF Info Page for EBF4 (EBF1)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain

Description

Description: early B-cell factor 4 [Source:HGNC Symbol;Acc:HGNC:29278]
Entrez Summary EBF4 belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors, members of which play important roles in neural development and B-cell maturation (Wang et al., 2002 [PubMed 12139918]).[supplied by OMIM, Mar 2008]
Ensembl ID: ENSG00000088881
External Link: CisBP
Interpro IPR002909; IPR014756; IPR018350; ;
Protein Domain: ENSP00000345030
Protein Domain: ENSP00000370022
Protein Domain: ENSP00000477107
Protein Domain: ENSP00000477023
Domain:
Protein: ENSP00000345030DBD: COE1 DBDOther: COE1_HLH, TIG
Protein: ENSP00000370022DBD: COE1 DBDOther: COE1_HLH, TIG
Protein: ENSP00000477107DBD: COE1 DBDOther:
Protein: ENSP00000477023DBD: COE1 DBDOther: COE1_HLH, TIG

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
b
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IBA - GO_REF:0000033
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredInferred - Ebf4 (96% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - EBF3 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - Ebf1 (83% AA Identity, Mus musculus)
MiscHOMERInferred - Ebf1 (83% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - Ebf2 (79% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - EBF2 (77% AA Identity, Homo sapiens)
HT-SELEXNitta2015Inferred - kn (72% AA Identity, Drosophila melanogaster)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution