TF Info Page for NFE2L1 (bZIP)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 2 Obligate heteromer In vivo/Misc source Only known motifs are from Transfac or HocoMoco - origin is uncertain Tested on HT-SELEX and PBM. Neither yielded a motif. Likely an obligate heteromer (PMID: 23661758).

Description

Description: nuclear factor, erythroid 2 like 1 [Source:HGNC Symbol;Acc:HGNC:7781]
Entrez Summary This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for
Ensembl ID: ENSG00000082641
External Link: CisBP
Interpro IPR004826; IPR004827; IPR008917; ;
Protein Domain: ENSP00000350072
Protein Domain: ENSP00000354855
Protein Domain: ENSP00000461960
Protein Domain: ENSP00000445811
Protein Domain: ENSP00000464446
Protein Domain: ENSP00000462003
Protein Domain: ENSP00000461971
Domain:
Protein: ENSP00000350072DBD: bZIPOther:
Protein: ENSP00000354855DBD: bZIPOther:
Protein: ENSP00000461960DBD: bZIPOther:
Protein: ENSP00000445811DBD: bZIPOther:
Protein: ENSP00000464446DBD: bZIPOther:
Protein: ENSP00000462003DBD: bZIPOther:
Protein: ENSP00000461971DBD: bZIPOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding
Transactivation_
PMIDS:8036168
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0001205
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involv
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
TAS - PMID:8248256
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
2a1, Lower confidence direct evidence
TF has conditional DNA-binding requirements Obligate_Multimer

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
TransfacTransfacLicense requiredInferred - NFE2L3 (65% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - NFE2L2 (60% AA Identity, Gallus gallus)
TransfacTransfacLicense requiredInferred - Nfe2l3 (60% AA Identity, Mus musculus)
HT-SELEXYin2017Inferred - NFE2 (58% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - NFE2 (58% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - NFE2 (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (58% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - NFE2 (58% AA Identity, Homo sapiens)
MiscHocoMocoInferred - NFE2 (58% AA Identity, Homo sapiens)
MiscHOMERInferred - NFE2 (58% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution