TF Info Page for TCF7 (HMG/Sox)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: transcription factor 7 (T-cell specific, HMG-box) [Source:HGNC Symbol;Acc:HGNC:11639]
Entrez Summary This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]
Ensembl ID: ENSG00000081059
External Link: CisBP
Interpro IPR009071; IPR013558;
Protein Domain: ENSP00000340347
Protein Domain: ENSP00000367822
Protein Domain: ENSP00000378469
Protein Domain: ENSP00000430179
Protein Domain: ENSP00000430617
Protein Domain: ENSP00000429935
Protein Domain: ENSP00000429547
Domain:
Protein: ENSP00000340347DBD: HMGOther: CTNNB1_binding
Protein: ENSP00000367822DBD: HMGOther: CTNNB1_binding
Protein: ENSP00000378469DBD: HMGOther: CTNNB1_binding
Protein: ENSP00000430179DBD: HMGOther: CTNNB1_binding
Protein: ENSP00000430617DBD: HMGOther: CTNNB1_binding
Protein: ENSP00000429935DBD: HMGOther:
Protein: ENSP00000429547DBD: HMGOther: CTNNB1_binding

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:7579399
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0001227
RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
SMiLE-seqIsakova2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
PBMBadis09Inferred - Tcf7 (100% AA Identity, Mus musculus)
PBMBadis09Inferred - Tcf7l2 (98% AA Identity, Mus musculus)
ChIP-seqJASPARInferred - TCF7L2 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TCF7L2 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TCF7L2 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TCF7L2 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TCF7L2 (98% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - TCF7L2 (98% AA Identity, Homo sapiens)
MiscHocoMocoInferred - TCF7L2 (98% AA Identity, Homo sapiens)
MiscHOMERInferred - TCF7L2 (98% AA Identity, Homo sapiens)
SMiLE-seqIsakova2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - LEF1 (97% AA Identity, Homo sapiens)
PBMBadis09Inferred - Lef1 (97% AA Identity, Mus musculus)
TransfacTransfacLicense requiredInferred - LEF1 (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - LEF1 (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - LEF1 (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - LEF1 (97% AA Identity, Homo sapiens)
MiscHocoMocoInferred - LEF1 (97% AA Identity, Homo sapiens)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution