TF Info Page for MEF2A (MADS box)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: Myocyte enhancer factor 2A [Source:HGNC Symbol;Acc:HGNC:6993]
Entrez Summary The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
Ensembl ID: ENSG00000068305
External Link: CisBP
Interpro IPR002100; IPR022102;
Protein Domain: ENSP00000346389
Protein Domain: ENSP00000337202
Protein Domain: ENSP00000454120
Protein Domain: ENSP00000452989
Domain:
Protein: ENSP00000346389DBD: MADS boxOther: HJURP_C
Protein: ENSP00000337202DBD: MADS boxOther: HJURP_C
Protein: ENSP00000454120DBD: MADS boxOther: HJURP_C
Protein: ENSP00000452989DBD: MADS boxOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:10748098, PMID:16484498, PMID:20590529
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IDA - PMID:7760790
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:16043483
GO:0003705
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
ChIP-seqENCODEDirect
SELEXJASPARDirect
ChIP-seqmodENCODEDirect
ChIP-seqmodENCODEDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERInferred - Mef2a (100% AA Identity, Mus musculus)
HT-SELEXJolma2010Inferred - MEF2C (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2C (97% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2C (97% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - MEF2C (97% AA Identity, Homo sapiens)
ChIP-seqJASPARInferred - MEF2C (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (97% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2C (97% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MEF2C (97% AA Identity, Homo sapiens)
MiscHOMERInferred - MEF2C (97% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2D (95% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2D (95% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - MEF2D (95% AA Identity, Homo sapiens)
MiscHocoMocoInferred - MEF2D (95% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2B (91% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - MEF2B (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2B (91% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - MEF2B (91% AA Identity, Homo sapiens)
ChIP-seqmodENCODEInferred - Mef2 (89% AA Identity, Drosophila melanogaster)
TransfacTransfacLicense requiredInferred - Mef2b (87% AA Identity, Mus musculus)

Structure

Structure PDB 1C7U

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution