TF Info Page for SPEN (Unknown)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Likely to be sequence specific TF 1 Monomer or homomultimer No motif Has a putative AT-hook Data inconclusive. Contains RRM domains, suggesting RNA binding activity. Gel-shift experiments demonstrate that SPEN (aka MINT) binds ss and dsDNA G- and T-rich elements (PMID: 10451362), but in another system SPEN does not bind DNA directly and is recruited (PMID: 15778499). RNA-binding activities have also been documented (PMID: 24748666).

Description

Description: spen family transcriptional repressor [Source:HGNC Symbol;Acc:HGNC:17575]
Entrez Summary This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000065526
External Link: CisBP
Interpro IPR000504; IPR010912; IPR012921; IPR016194;
Protein Domain:
Domain:
Protein: DBD: OtherOther:

Previous Annotations

Source Annotation
TF-CAT classification TF Gene Candidate_Single stranded RNA/DNA binding_DNA Binding_
PMIDS:10451362 15778499
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
No
CisBP considers it as a TF? No
TFclass considers it as a TF? No
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" No
GO-Info
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
5a, one of the source datasets predicts is as a TF
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution