TF Info Page for POU1F1 (Homeodomain; POU)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro

Description

Description: POU class 1 homeobox 1 [Source:HGNC Symbol;Acc:HGNC:9210]
Entrez Summary This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000064835
External Link: CisBP
Interpro IPR000327; IPR001356; IPR009057; IPR010982; IPR013847; IPR017970;
Protein Domain: ENSP00000263781
Protein Domain: ENSP00000342931
Protein Domain: ENSP00000452610
Protein Domain: ENSP00000454072
Domain:
Protein: ENSP00000263781DBD: Homeodomain,POUOther:
Protein: ENSP00000342931DBD: Homeodomain,POUOther:
Protein: ENSP00000452610DBD: Homeodomain,POUOther:
Protein: ENSP00000454072DBD: Homeodomain,POUOther:

Previous Annotations

Source Annotation
TF-CAT classification No
PMIDS:
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0000981
sequence-specific DNA binding RNA polymerase II transcription factor activity
IDA - PMID:26612202
GO:0001077
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor a
IEA - GO_REF:0000019
GO:0003700
sequence-specific DNA binding transcription factor activity
IDA - PMID:11301317, PMID:9482665
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
TransfacTransfacLicense requiredDirect
MiscHocoMocoDirect
MiscHOMERInferred - Pou1f1 (97% AA Identity, Rattus norvegicus)
MiscHOMERInferred - Pou1f1 (97% AA Identity, Rattus norvegicus)
PBMBerger08Inferred - Pou1f1 (96% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Pou1f1 (96% AA Identity, Mus musculus)
PBMDREAM_contestInferred - Pou1f1 (95% AA Identity, Mus musculus)
PBMTF_EvolutionUnpublished DataInferred - NEMVEDRAFT_v1g112929 (78% AA Identity, Nematostella vectensis)

Structure

Structure PDB 1AU7

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution