The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	heat shock transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:5225]
Entrez Summary	TBA
Ensembl ID:	ENSG00000025156
External Link:
Interpro	IPR000232; IPR010542;
Protein Domain:
Protein: ENSP00000357440	DBD: HSF	Other: Vert_HS_TF
Protein: ENSP00000400380	DBD: HSF	Other: Vert_HS_TF
Protein: ENSP00000477405	DBD: HSF	Other: Vert_HS_TF

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	Yes
GO-Info	GO:0001228 RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor IDA - PMID:10561509 GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:1871106
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2010		Direct
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Transfac	Transfac	License required	Direct
Misc	HocoMoco		Direct
PBM	Zoo_01		Inferred - hsf2 (90% AA Identity, Danio rerio)

Structure

Structure	PDB	5D8K

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 110, "endStyle": "curved", "aliStart": 10, "text": "HSF", "colour": "#009900", "aliEnd": 110, "start": 10, "href": "http://pfam.xfam.org/family/PF00447.15", "type": "pfama", "display": "true", "metadata": {"end": 110, "description": "Heat shock factor (HSF) is a transcriptional activator of heat shock genes [PUBMED:2257625]: it binds specifically to heat shock promoter elements, which are palindromic sequences rich with repetitive purine and pyrimidine motifs [PUBMED:2257625].", "database": "PfamA", "aliStart": 10, "scoreName": "E-value", "accession": "PF00447.15", "start": 10, "score": 9.3e-33, "identifier": "HSF-type DNA-binding", "type": "DBD", "aliEnd": 110}}, {"startStyle": "straight", "end": 393, "endStyle": "jagged", "aliStart": 230, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 392, "start": 230, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 393, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 230, "scoreName": "E-value", "accession": "PF06546.9", "start": 230, "score": 1e-57, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 392}}, {"startStyle": "jagged", "end": 495, "endStyle": "straight", "aliStart": 394, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 493, "start": 393, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 495, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 394, "scoreName": "E-value", "accession": "PF06546.9", "start": 393, "score": 1e-57, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 493}}], "length": 519}

{"regions": [{"startStyle": "straight", "end": 164, "endStyle": "jagged", "aliStart": 1, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 163, "start": 1, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 164, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 1, "scoreName": "E-value", "accession": "PF06546.9", "start": 1, "score": 1.1999999999999999e-52, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 163}}, {"startStyle": "jagged", "end": 214, "endStyle": "jagged", "aliStart": 165, "text": "Vert_HS_TF", "colour": "#9999ff", "aliEnd": 210, "start": 164, "href": "http://pfam.xfam.org/family/PF06546.9", "type": "pfama", "display": "true", "metadata": {"end": 214, "description": "This family represents the C-terminal region of vertebrate heat shock transcription factors. Heat shock transcription factors regulate the expression of heat shock proteins - a set of proteins that protect the cell from damage caused by stress and aid the cell's recovery after the removal of stress [1]. This C-terminal region is found with the N-terminal Pfam:PF00447, and may contain a three-stranded coiled-coil trimerisation domain and a CE2 regulatory region, the latter of which is  involved in sustained heat shock response [1].", "database": "PfamA", "aliStart": 165, "scoreName": "E-value", "accession": "PF06546.9", "start": 164, "score": 1.1999999999999999e-52, "identifier": "Vertebrate heat shock transcription factor", "type": "DBD", "aliEnd": 210}}], "length": 214}

TF Info Page for HSF2 (HSF)