TF Info Page for E2F2 (E2F)

Conclusion

Assessment Binding Mode Motif Status Notes Comments
Known motif 1 Monomer or homomultimer High-throughput in vitro Can also bind as a heterodimer with DP1 (PMID: 16360038)

Description

Description: E2F transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:3114]
Entrez Summary The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]
Ensembl ID: ENSG00000007968
External Link: CisBP
Interpro IPR003316; ;
Protein Domain: ENSP00000355249
Domain:
Protein: ENSP00000355249DBD: E2FOther: E2F_CC-MB

Previous Annotations

Source Annotation
TF-CAT classification TF Gene_DNA-Binding
sequence-specific_DNA Binding_
PMIDS:10766737 11883935
Vaquerizas 2009 TF classification
"a" Has direct evidence of TF function;
"b" Has evidence for an orthologous TF;
"c" contains likely DBDs, but has no functional evidence;
"x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription;
"other" category contains proteins without clear DBDs they curated from external sources.
a
CisBP considers it as a TF? Yes
TFclass considers it as a TF? Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding" Yes
GO-Info GO:0003700
sequence-specific DNA binding transcription factor activity
IEA - GO_REF:0000019
Initial Assessment
1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding;
1a2 There is high confidence data for a close ortholog (as defined in CisBP);
2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model;
2a2 There is lower confidence evidence for an close ortholog;
3a There is decent circumstantial evidence for its role as a TF or not;
4a Two or more datasets predict it as a TF;
5a One of the source datasets predicts is as a TF
1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source Annotation Motif Evidence
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXJolma2013Direct
HT-SELEXYin2017Direct
HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
Methyl-HT-SELEXYin2017Direct
PBMZoo_01Direct
MiscHocoMocoDirect
PBMBadis09Inferred - E2f2 (98% AA Identity, Mus musculus)
PBMZoo_01Inferred - E2F2 (96% AA Identity, Tupaia belangeri)
PBMZoo_01Inferred - E2F2 (92% AA Identity, Anolis carolinensis)
PBMZoo_01Inferred - ENSOANG00000014967 (89% AA Identity, Ornithorhynchus anatinus)
PBMZoo_01Inferred - E2F3 (86% AA Identity, Taeniopygia guttata)
HT-SELEXYin2017Inferred - E2F3 (84% AA Identity, Homo sapiens)
Methyl-HT-SELEXYin2017Inferred - E2F3 (84% AA Identity, Homo sapiens)
PBMBadis09Inferred - E2f3 (84% AA Identity, Mus musculus)
SELEXJASPARInferred - E2F3 (84% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F3 (84% AA Identity, Homo sapiens)
MiscHocoMocoInferred - E2F3 (84% AA Identity, Homo sapiens)
HT-SELEXYin2017Inferred - E2F1 (78% AA Identity, Homo sapiens)
ChIP-seqENCODEInferred - E2F1 (78% AA Identity, Homo sapiens)
SELEXJASPARInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
TransfacTransfacLicense requiredInferred - E2F1 (78% AA Identity, Homo sapiens)
MiscHocoMocoInferred - E2F1 (78% AA Identity, Homo sapiens)
MiscHOMERInferred - E2F1 (78% AA Identity, Homo sapiens)
PBMTF_EvolutionUnpublished DataInferred - estExt_gwp_gw1.C_220046 (75% AA Identity, Physcomitrella patens)
PBMZoo_01Inferred - e_gw1.38.2.1 (73% AA Identity, Physcomitrella patens)
Dap-seqOMalley2016Inferred - E2FC (72% AA Identity, Arabidopsis thaliana)
ChIP-seqmodENCODEInferred - E2f (70% AA Identity, Drosophila melanogaster)
Dap-seqOMalley2016Inferred - E2FA (70% AA Identity, Arabidopsis thaliana)
Dap-seqOMalley2016Inferred - E2FA (70% AA Identity, Arabidopsis thaliana)

Structure

Structure PDB Not_Covered

Experimental History

Method Constructs
Tried in PBM?
(Whether the protein was tried in PBM or not)
Tried in HT-SELEX
(Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information?
(Tried with another method and failed?)

External Contribution