The Human Transcription Factors

Conclusion

Assessment	Binding Mode	Motif Status	Notes	Comments
Known motif	1 Monomer or homomultimer	High-throughput in vitro

Description

Description:	Distal-less homeobox 6 [Source:HGNC Symbol;Acc:HGNC:2919]
Entrez Summary	TBA
Ensembl ID:	ENSG00000006377
External Link:	T093227_1.02
Interpro	IPR000047; IPR001356; IPR009057; IPR017970; IPR020479; ;
Protein Domain:
Protein: ENSP00000428480	DBD: Homeodomain	Other: RskA
Protein: ENSP00000451635	DBD: Homeodomain	Other:

Previous Annotations

Source	Annotation
TF-CAT classification	No PMIDS:
Vaquerizas 2009 TF classification "a" Has direct evidence of TF function; "b" Has evidence for an orthologous TF; "c" contains likely DBDs, but has no functional evidence; "x" is an unlikely TF such as predicted gene, genes with likely non-specific DBDs or that have function outside transcription; "other" category contains proteins without clear DBDs they curated from external sources.	a
CisBP considers it as a TF?	Yes
TFclass considers it as a TF?	Yes
Has GO:0003700 "transcription factor activity, sequence-specific DNA binding"	No
GO-Info	GO:0003700 sequence-specific DNA binding transcription factor activity TAS - PMID:7907794
Initial Assessment 1a1 Protein has a high confidence PWM (HT-SELEX, PBM or B1H model) or there is a crystal structure that supports sequence specific DNA binding; 1a2 There is high confidence data for a close ortholog (as defined in CisBP); 2a1 There is lower confidence direct evidence, such as a Jaspar, Hocomoco or Transfac model; 2a2 There is lower confidence evidence for an close ortholog; 3a There is decent circumstantial evidence for its role as a TF or not; 4a Two or more datasets predict it as a TF; 5a One of the source datasets predicts is as a TF	1a1, Direct HQ evidence
TF has conditional DNA-binding requirements

DNA-Binding

Published Motif Data

Source	Annotation	Motif	Evidence
HT-SELEX	Jolma2013		Direct
HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
Methyl-HT-SELEX	Yin2017		Direct
HT-SELEX	Yin2017		Inferred - DLX1 (98% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX1 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX1 (98% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX1 (98% AA Identity, Homo sapiens)
PBM	Berger08		Inferred - Dlx1 (98% AA Identity, Mus musculus)
B1H	JASPAR		Inferred - Dll (91% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Dll (91% AA Identity, Drosophila melanogaster)
HT-SELEX	Nitta2015		Inferred - Dll (91% AA Identity, Drosophila melanogaster)
PBM	Zoo_01		Inferred - Dll (91% AA Identity, Drosophila melanogaster)
HT-SELEX	Yin2017		Inferred - DLX2 (89% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX2 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX2 (89% AA Identity, Homo sapiens)
PBM	Berger08		Inferred - Dlx2 (89% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX4 (89% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX5 (89% AA Identity, Homo sapiens)
PBM	Berger08		Inferred - Dlx5 (89% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - DLX5 (89% AA Identity, Homo sapiens)
Misc	HocoMoco		Inferred - DLX2 (89% AA Identity, Homo sapiens)
PBM	Berger08		Inferred - Dlx4 (87% AA Identity, Mus musculus)
HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
Methyl-HT-SELEX	Yin2017		Inferred - DLX3 (85% AA Identity, Homo sapiens)
PBM	Berger08		Inferred - Dlx3 (85% AA Identity, Mus musculus)
Transfac	Transfac	License required	Inferred - DLX3 (85% AA Identity, Homo sapiens)
Transfac	Transfac	License required	Inferred - P53774 (85% AA Identity, Xenopus laevis)
Misc	HocoMoco		Inferred - DLX3 (85% AA Identity, Homo sapiens)

Structure

Structure	PDB	Not_Covered

Experimental History

Method	Constructs
Tried in PBM? (Whether the protein was tried in PBM or not)
Tried in HT-SELEX (Whether the protein was tried in HT-SELEX or not, and if so, then what kind of clones were tested)
Other Information? (Tried with another method and failed?)

External Contribution

Name
Email
Comment
Reference

{"regions": [{"startStyle": "curved", "end": 224, "endStyle": "curved", "aliStart": 168, "text": "HD", "colour": "#228B22", "aliEnd": 224, "start": 168, "href": "http://pfam.xfam.org/family/PF00046.27", "type": "pfama", "display": "true", "metadata": {"end": 224, "description": "The homeobox domain or homeodomain was first identified in a number of drosophila homeotic and segmentation proteins, but is now known to be well-conserved in many other animals, including vertebrates [PUBMED:2568852, PUBMED:1357790]. Hox genes encode homeodomain-containing transcriptional regulators that operate differential genetic programs along the anterior-posterior axis of animal bodies [PUBMED:12445403]. The domain binds DNA through a helix-turn-helix (HTH) structure. ", "database": "PfamA", "aliStart": 168, "scoreName": "E-value", "accession": "PF00046.27", "start": 168, "score": 1.3000000000000001e-20, "identifier": "Homeobox domain", "type": "DBD", "aliEnd": 224}}, {"startStyle": "straight", "end": 182, "endStyle": "jagged", "aliStart": 35, "text": "RskA", "colour": "#9999ff", "aliEnd": 150, "start": 26, "href": "http://pfam.xfam.org/family/PF10099.7", "type": "pfama", "display": "true", "metadata": {"end": 182, "description": "This domain, formerly known as DUF2337, is the anti-sigma-K factor, RskA. In Mycobacterium tuberculosis the protein positively regulates expression of the antigenic proteins MPB70 and MPB83 [1].", "database": "PfamA", "aliStart": 35, "scoreName": "E-value", "accession": "PF10099.7", "start": 26, "score": 0.00074, "identifier": "Anti-sigma-K factor rskA", "type": "DBD", "aliEnd": 150}}], "length": 294}

TF Info Page for DLX6 (Homeodomain)