Collection of 30 putative HMG/Sox human TFs out of 57 considered proteins

DBD Alignment (Click to open/enlarge)


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No Structure and Description available
Ensembl ID HGNC symbol DBD Family Is TF? TF Assessment Binding Mode Motif Status Notes Comments
AC105001.2 HMG/Sox No Unlikely to be sequence specific TF 4 Not a DNA binding protein No motif Unlikely to be sequence specific TF - it has a deletion inside the SOX-domain removing several residues that contact DNA based on the structure of paralog SOX17.
BBX HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
CCDC124 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Based on the alignment, it lacks a proper HMG domain. Evidence it binds RNA (PMID: 22658674).
CHD3 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Has a putative AT-hook No evidence for sequence-specific DNA-binding in the literature; and contains only a partial match to an HMG box with no similarity to DBDs of other CHD family proteins
CHD4 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif CHD4 is likely not a sequence-specific DNA-binding protein despite having a partial match to an HMG box. This protein binds to the DNA backbone, but binds poly(ADP-ribose) with higher affinity (PMID: 26565020).
CIC HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
HBP1 HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
HMG20A HMG/Sox Yes Likely to be sequence specific TF 1 Monomer or homomultimer No motif Similar to HMG20B, which has an inferred motif from mouse.
HMG20B HMG/Sox Yes Inferred motif 1 Monomer or homomultimer High-throughput in vitro
HMGB1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs. Evidence exists showing HMGB1/2 are non-sequence-specific binding proteins (PMID: 11497996).
HMGB2 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs. Evidence exists showing HMGB1/2 are non-sequence-specific binding proteins (PMID: 11497996). HMGB2 is well-known to facilitate DNA-bending and interact with sequence-specific TFs and transcriptional co-factors.
HMGB3 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Similar to HMGB1/2, which bind DNA with low specificity
HMGB4 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Similar to HMGB1/2, which bind DNA with low specificity. Yet, it can also bind with 4.35nM affinity to DNA containing an intra-strand crosslink made by cis-platin (PMID: 22901013).
HMGN1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs.
HMGN3 HMG/Sox Yes Likely to be sequence specific TF 3 Low specificity DNA-binding protein No motif
HMGXB3 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs.
HMGXB4 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs.
KMT2C HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Has a putative AT-hook Based on the alignment, it is quite different from all other HMG proteins. It is borderline to say that it is a HMG at all.
KMT2D HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Based on alignment it is quite different from all other HMG proteins, borderline to say if it is a HMG at all. Crystal structure has an aminoacid sequence that doesnt match to the protein it claims to be
LEF1 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
MAEL HMG/Sox No ssDNA/RNA binding 4 Not a DNA binding protein No motif Orthologous protein from mouse binds RNA (PMID: 25807393)
NSD2 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Likely affects transcription by histone modification (not specific DNA-binding).
PBRM1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif (PMID: 23867514) shows it may bind nucleosomal DNA.
PMS1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs.
SMARCE1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif In the alignment, it clusters with other HMG proteins that have not yielded motifs. Contributes to SWI/SNF DNA-binding (PMID: 9435219).
SOX1 HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
SOX10 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX11 HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
SOX12 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX13 HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
SOX14 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX15 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX17 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX18 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX2 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX21 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX3 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX30 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX4 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro Also binds ssDNA loops.
SOX5 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX6 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX7 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX8 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SOX9 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro Also binds ssDNA loops.
SRY HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
SSRP1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif HMG domain is highly divergent based on AA-sequence
TCF7 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
TCF7L1 HMG/Sox Yes Known motif 1 Monomer or homomultimer High-throughput in vitro
TCF7L2 HMG/Sox Yes Known motif 1 Monomer or homomultimer 100 perc ID - in vitro
TFAM HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif HMG domain is highly divergent based on AA-sequence
TOX HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Predicted to be a non- or low specificity TF (PMID: 12697058). However, there is a DAM-id based motif with SSSSGNNGCG-consensus (PMID: 25527292), but this does not look like HMG-site
TOX2 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif TOX proteins are clearly their own distinct subfamily of HMG proteins. No strong evidence for them binding DNA specifically.
TOX3 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif TOX proteins are clearly their own distinct subfamily of HMG proteins. No strong evidence for them binding DNA specifically.
TOX4 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif TOX proteins are clearly their own distinct subfamily of HMG proteins. No strong evidence for them binding DNA specifically.
UBTF HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif No evidence for or against specific DNA-binding by this class of HMG box containing proteins
UBTFL1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif No evidence for or against specific DNA-binding by this class of HMG box containing proteins
WDHD1 HMG/Sox No Unlikely to be sequence specific TF 3 Low specificity DNA-binding protein No motif Divergent HMG motif, doesnt cluster either with specific or nonspecific HMG proteins